5T4 Rabbit Monoclonal Antibody for WB and ELISA - Q13641

Core Product Specifications and Parameters

Biological Background: Trophoblast Glycoprotein Function and Localization

• Trophoblast glycoprotein (5T4, also known as M6P1, WAIF1) is encoded by the TPBG gene and is a single-pass type I membrane protein featuring N-terminal leucine-rich repeats (LRRs) and a signal peptide.
• It acts as an inhibitor of Wnt/β-catenin signaling by indirectly binding to LRP6 and blocking Wnt3a-induced LRP6 internalization, impacting cell fate and proliferation.
• 5T4 is prominently expressed in placental trophoblast cells from early gestation and is highly upregulated in various carcinomas, while showing minimal expression in most normal adult tissues.
• The protein is N-glycosylated and contains disulfide bonds that stabilize its extracellular domain structure.
• Phosphorylation of 5T4 may modulate its interaction with signaling partners.
• Given its restricted oncofetal distribution and role in Wnt pathway modulation, 5T4 is explored as a target in cancer immunotherapy and diagnostic development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 321-420) of human 5T4, which is accessible in both native and denatured conditions, supporting use in Western blotting under reducing and non-reducing protocols.
• For ELISA applications, consider coating with recombinant 5T4 extracellular domain or using lysates from human trophoblast-derived or 5T4-positive carcinoma cell lines to ensure specificity.
• Validate antibody reactivity in the specific tissue or cell type of interest, as expression may vary among cancer models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers, offering validated antibodies, antigen pairs, and bulk ancillary reagents. For 5T4-related oncology and developmental biology research, we provide monoclonal antibodies and customized raw-material solutions, supporting your projects from initial development to clinical application.